As CityLink undergoes the final stages of the M1 Upgrade Project, there will be several lane closures heading inbound on CityLink between Yarra Boulevard and Punt Road from 28-30 January.
Over the weekend, expansion joints will be replaced with new asphalt, and line marking will also take place.
The work must be completed in warmer weather but was deliberately held until after the busy Christmas period.
CityLink has warned drivers heading towards the city during the day on Saturday and Sunday to expect long delays.